Aquaporin 4 Monoclonal Antibody(4H1)

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Home > Product > Antibody > Aquaporin 4 Monoclonal Antibody(4H1)

Aquaporin 4 Monoclonal Antibody(4H1)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB, IHC-p, IF, ICC

Product overview

Full product name

Aquaporin-4

Code

BT-MCA0007

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Aquaporin 4

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

34830

Gene ID

Human: 361; Mouse: 11829; Rat: 25293

Synonyms

AQP4; Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4

Research area

Signal transduction

Target protein

AQP4

Recommended dilution

Application	Dilution
IF	1:100-200
IHC	1:50-300
WB	1:1000

Images

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1.Aquaporin 4 Monoclonal antibody(4H1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-appendix tissue. 1.Aquaporin 4 Monoclonal antibody(4H1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-brain tissue. 1.Aquaporin 4 Monoclonal antibody(4H1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue diluted at 1:2000.

IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

Western blot analysis of lysates from 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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